【文献标题】Therapeutic effect of human amniotic epithelial cells in murine models of Hashimoto’s thyroiditis and Systemic lupus erythematosus
【作者】BING TAN，PENGJIE YANG，JINYING LI，et.al
【关键词】cell therapy, experimental autoimmune thyroiditis, human amniotic epithelial cells, Hashimoto’s thyroiditis，immunomodulatory, Systemic lupus erythematosus
Sera cytokine levels of IL-17A, IFN-γ, IL-4, IL-10 and TGF-β of EAT and SLE mice were measured by ELISA. All ELISA kits were from Hengyuan Biological Technologies (Shanghai, China). Specific experimental steps were performed according to the manufacturer’s instructions.
Cytokine production in vitro
Splenocytes of EAT mice at the peak of disease (35 day) were stimulated with pTg in the presence or absence of hAECs. Cell-free culture supernatants were collected after 48 h by centrifugation. Cytokine levels of IL-17A, IFN-γ,IL-4, IL-10 and TGF-β were measured by ELISA(Hengyuan BiologicalTechnologies, Shanghai, China).